A solution of tris(hydroxymethyl)aminomethane, salt, and other components prepared according to a specific formulation is commonly used in biological and biochemical applications. This formulated solution, designed to maintain a stable pH, provides an isotonic environment suitable for cells and sensitive biomolecules. For example, it’s often employed as a wash buffer in immunoassays or as a diluent for protein samples.
The consistent pH and ionic strength afforded by such a solution are critical for preserving the integrity and activity of biological materials during experimental procedures. Its use minimizes degradation, denaturation, or aggregation of proteins and nucleic acids. Historically, the adoption of similar buffering systems significantly improved the reproducibility and reliability of biological experiments, enabling advancements in various fields of scientific research.